PGs Board Game

15

27

43

19

39

35

11

23

31

46

30

34

38

42

26

18

22

10

14

24

44

16

28

32

20

12

36

40

25

17

45

13

37

21

41

29

33

How many PGs can you produce?

start

Drag the pieces to start

Teams

end

Prostaglandins count

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Mini game

+2

Due to the congress in which you are participating next week you are trying to learn the vocabulary related to this project. Play Hangman using this vocabulary.

Protocol

+5

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You want to perform a DNase digestion on your RNA sample containing 7.5 µL. If the total volume of the reaction should be 50 µL, how many µL of water should you add if you add 1µL of enzyme and 5µL of buffer?

36.5 µL of water.

+3

+4

Dare

Truth

Find at least 3 words related to the project in the word search:

+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

Mini game

+2

Due to the congress in which you are participating next week you are trying to learn the vocabulary related to this project. Play Hangman using this vocabulary.

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+2

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+2

Your cells feel happy and motivated.

You win 3 PGs.

You have done an excellent job counting your cells and refreshing the medium every 5 days.

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+2

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Mini challenge

+8

One of the treatments in your experiment is to stress your diatoms by growing them at 25°C. However, you have found it difficult to maintain the culture at this temperature. Using the materials around you, create a way to raise the temperature of your culture to 25°C.

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Mini challenge

+4

You were refreshing your cultures with new media, but you were starving and left it half done. Now your cells are in danger of being contaminated if in the next 30 seconds you don't put on your lab coat and get back to work. Can you do it?

You want to prepare 2 mL of 80% Ethanol. How much volume of water and how much volume of ethanol would you add?

400 μL of water.1600 μL of ethanol.

+3

+3

Dare

Truth

Find at least 3 words related to the project in the word search:

+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

Your cells feel happy and motivated.

You win 3 PGs.

You have done an excellent job counting your cells and refreshing the medium every 5 days.

Your cells feel happy and motivated.

You win 3 PGs.

You have done an excellent job counting your cells and refreshing the medium every 5 days.

Your cells were overstressed and died.

You lose 3 PGs.

Remember that it is important to refresh your culture every 5 days.

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Your cells feel happy and motivated.

You win 3 PGs.

You have done an excellent job counting your cells and refreshing the medium every 5 days.

+2

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According to the results showed in the following gel, is the RNA extracted of a good quality?

The RNA shows good quality.

+4

+3

Dare

Truth

Your grandmother has always liked science, so every time that she sees you, she asks you about your project. Considering that she didn't study Marine Sciences, how would you explain to her in a simple way what a diatom is?

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+2

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Primers forward and reverse, template (sample), dNTPs, DNA polymerase, and reaction buffer.

+2

+5

Dare

Truth

Due to the congress in which you are participating next week you are trying to learn the vocabulary related to this project.Play Hangman using this vocabulary.

Name the 5 reagents needed for a PCR:

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Mini game

+2

Due to the congress in which you are participating next week you are trying to learn the vocabulary related to this project. Play Hangman using this vocabulary.

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+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

Considering that the expected band size representing contamination of the RNA sample with gDNA using RPS primers is 100bp, are any of the samples showed in the following gel contaminated?

8, 10 and 12

+4

Truth

+5

Dare

Your grandmother has always liked science, so every time that she sees you, she asks you about your project. Considering that she didn't study Marine Sciences, how would you explain to her in a simple way what a diatom is?

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You want to perform a DNase digestion on your RNA sample containing 8.2 µL. If the total volume of the reaction should be 50 µL, how many µL of water should you add if you add 1µL of enzyme and 5µL of buffer?

35.8 µL of water.

+4

+4

Dare

Truth

Your diatom culture is not growing properly and you think it's because your cells are bored, therefore you decide to dance to entertain them. Dance Macarena to earn 4 PGs.

+2

You want to prepare a master mix for 9 PCR reactions. The total volume per reaction is 25 μL, adding 0.7 μL of each primer, 1 μL of sample (template) and 12.5 μL of TaqMix. How many μL of water would you add and how much would be the total volume you would add to the Master mix for each reagent?

10.1 μL of water.0.7 x9 = 6.3 μL for each primer. 12.5 x9 = 112.5 μL of TaqMix 10.1 x9 = 90.9 μL of water.

+5

+7

Dare

Truth

Throughout the development of your project your cells have been growing as expected. Therefore, you are very grateful to them and have decided to compose them a poem that rhymes with the name of their species: Thalassiosira rotula Use the next minutes to get inspired and compose a short poem.

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Mini challenge

+8

One of the treatments in your experiment is to stress your diatoms by growing them at 10°C. However, you have found it difficult to maintain the culture at this temperature. Using the materials around you, create a way to lower the temperature of your culture to 10°C.

Dare

During your PCR preparation you want to add 12.5 μL of TaqMix. Which micropipette and tips would you use?

#1 and #5

+4

+3

Truth

Your diatom culture is not growing properly and you think it's because your cells are bored, therefore you decide to dance to entertain them. Dance Dancing Queen to earn 4 PGs.

Your cells were overstressed and died.

You lose 3 PGs.

Remember that it is important to refresh your culture every 5 days.

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Mini challenge

+6

You are trying to explain this project to some rappers but they can't understand you. Make some rhymes using at least the words "diatom", "Biotechnology", "prostaglandin", and "RNA" for them. Be as creative as you can.

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+2

Protocol

+5

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+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

Primers forward and reverse, template (sample), dNTPs, DNA polymerase, and reaction buffer.

+4

+3

Dare

Truth

Due to a budget cut there are not enough resources to buy more 1 mL micropipette tips. Because you have already started your RNA extraction, you have 30 seconds to look around and find someone who can donate you a box.

Name 3 of the reagents needed for a PCR:

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Your cells were overstressed and died.

You lose 3 PGs.

Remember that it is important to refresh your culture every 5 days.

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+2

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Your supervisor has asked you the name of the species of diatom that you are working with, however he has problems hearing and could not understand you. Now you have to shout 5 times "Thalassiosira rotula" so hopefully he could understand. Are you ready?

According to the results showed in the following gel, is the RNA extracted of a good quality?

The RNA doesn't have good quality.

+5

+3

Dare

Truth

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Dare

For a PCR reaction of 25 μL total volume, calculate the volume of water needed according to the following list: TaqMix 12.5 μL, Primer F 0.7 μL, Primer R 0.7 μL, template 1 μL

10.1μL

+5

+4

Truth

Your supervisor has asked you the name of the species of diatom that you are working with, however he has problems hearing and could not understand you. Now you have to shout 5 times "Thalassiosira rotula" so hopefully he could understand. Are you ready?

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+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

+2

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+2

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Mini game

+2

Due to the congress in which you are participating next week you are trying to learn the vocabulary related to this project. Play Hangman using this vocabulary.

+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

Lorem ipsum dolor

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod.

• Lorem ipsum dolor sit amet.
• Consectetur adipiscing elit.
• Sed do eiusmod tempor incididunt ut.
• Labore et dolore magna aliqua.

+3

How many cells are here?

Count the cells and after place the cursor on the PG to reveal the correct answer.

100

During your RNA extraction you want to add 750 μL of TRIS. Which micropipette and tips would you use?

#3 + #1

+3

+3

Dare

Truth

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