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Paraffin
Resin
Embedding/block
Fixation
2
1
Formaldehyde 10%
Ethanol 70%
Methanol 100%
Freezing
Glutaraldehyde
Histological Processing of tissue samples
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Histological Processing of tissue samples

Formaldehyde 10%Ethanol 70%Methanol 100%FreezingGlutaraldehyde

Fixation

Embedding/block

cutting

staining

mounting

Observation

ParaffinResin

Paraffin microtome CryostatUltramicrotome

Hematoxylin-Eosin (H-E)PASTrichromic stainingSilver stainingNissl

Mounting in adhesive media

Optic microscopeFluorescence microscopeElectronic microscope

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https://youtu.be/N96x3Xtp95w

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0: Instructions

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Please, navigate into this interactive page to get general information. Group A will foccus on the protocol for liquid samples Group B will foccus on the protocol for solid samples Finally, elaborate your own detailed protocol.

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