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Histological processing
María Alemán
Created on June 22, 2023
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Transcript
Histological Processing of tissue samples
Formaldehyde 10%Ethanol 70%Methanol 100%FreezingGlutaraldehyde
Fixation
Embedding/block
cutting
staining
mounting
Observation
ParaffinResin
Paraffin microtome CryostatUltramicrotome
Hematoxylin-Eosin (H-E)PASTrichromic stainingSilver stainingNissl
Mounting in adhesive media
Optic microscopeFluorescence microscopeElectronic microscope
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https://youtu.be/N96x3Xtp95w
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0: Instructions
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Please, navigate into this interactive page to get general information. Group A will foccus on the protocol for liquid samples Group B will foccus on the protocol for solid samples Finally, elaborate your own detailed protocol.