FBXW7

START

Advanced Biochemistry Dr. Fishovitz Kellie Krohn

Protein Project: FBXW7

Why should we study cancer?

+ info

Around 2,000,000 Americans are diagnosed with cancer each year. Around 600,000 Americans will pass away due to cancer in just one year.

Studying proteins may increase our understanding on what mechanisms drive cancer

Research

F-Box W7 Protein

belonging to the Skp1-Cdc53/Cullin-F-box-protein complex (SCF/β-TrCP)- F-Box proteins- regulate proteins involved in DNA replication, transcription, cell differentiation, and cell death, via ubiquitination-mediated degradation of target proteins (Kahn, 2021).

FBXW7 function

often inactivated or mutated in humans- correlates with:- cancer progression- therapy resistance - poor prognosis Indicated as a potential target for treatment for many cancers

FBXW7 role in humans

Proliferation Assay- How a molecule may affect a cells proliferation, growth or viability
Wound Scratch Assay- How a molecule affects migration of the cells
Chemotaxis Assay- Whether cells migrate towards a particular agent

Cellular Assays

Research Techniques:

Endometrial Cancer Cell Lines

Scratch WoundAssay

Chemotaxis Assay

Proliferation assay

figure

Additional cell lines: HEC-1-B, HEC-1-A, KLE, SKOV3shRNA knockouts, western blots

Future Directions with studying FBXW7

The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

FBXW7 role in:- Notch Signaling Pathway and Hepatic Fibrosis- Colorectal Cancer

Current Research on FBXW7

Eureka!

Thank you!!

Cuevas, I. C.; Sahoo, S. S.; Kumar, A.; Zhang, H.; Westcott, J.; Aguilar, M.; Cortez, J. D.; Sullivan, S. A.; Xing, C.; Hayes, D. N.; Brekken, R. A.; Bae-Jump, V. L.; Castrillon, D. H. Fbxw7 Is a Driver of Uterine Carcinosarcoma by Promoting Epithelial-Mesenchymal Transition. Proceedings of the National Academy of Sciences 2019, 116 (51), 25880-25890. https://doi.org/10.1073/pnas.1911310116.Liu, Y., Chen, H., Bao, H., Zhang, J., Wu, R., & Zhu, L. (2023). Comprehensive characterization of FBXW7 mutational and clinicopathological profiles in human colorectal cancers. Frontiers in oncology, 13, 1154432. https://doi.org/10.3389/fonc.2023.1154432Sun, Y., He, L., Guo, P., Li, F., Wang, B., Zhang, Y., An, K., & Peng, M. (2023). F-box and WD repeat domain containing 7 inhibits the activation of hepatic stellate cells by degrading delta-like ligand 1 to block Notch signaling pathway. Open medicine (Warsaw, Poland), 18(1), 20230634. https://doi.org/10.1515/med-2023-0634

Refrences

Lorem ipsum dolor sit amet, consectetuer

Calendar (Month)

Expetenda tincidunt in sed, ex partem placerat sea, porro commodo ex eam. His putant aeterno interesset at. Usu ea mundi tincidunt, omnium virtute aliquando ius ex. Ea aperiri sententiae duo. Usu nullam dolorum quaestio ei, sit vidit facilisis ea. Per ne impedit iracundia neglegentur.

Calendar (Trimester)

Lorem ipsum dolor sit amet

Lorem ipsum dolor

Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper.

Lorem ipsum

Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat.

+ info

Lorem ipsum

Lorem ipsum dolor sit amet

Est ei erat mucius quaeque. Ei his quas phaedrum, efficiantur mediocritatem ne sed, hinc oratio blandit ei sed blandit.

Step 03

His audiam deserunt in, eum ubique voluptatibus ten reque dicta usu. Ne rebum dissentiet eam, vim omnis deseruisse.

Step 02

Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut.

Step 01

Process

+ info

Lorem ipsum dolor sit amet

Lorem ipsum dolor sit

Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut wisi enim ad minim veniam, quis nostrud exerci

Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut.

+ info

Lorem ipsum dolor sit amet

Process

Step 4

Ullamcorper suscipit lobortis nisl ut aliquip

Step 3

Duis autem vel eum irem dolor in hendrerit

Ullamcorper suscipit lobortis nisl ut aliquip

Duis autem vel eum irem dolor in hendrerit

Step 2

Step 1

+ info

Step 4

Ullamcorper suscipit lobortis nisl ut aliquip

Step 3

Duis autem vel eum irem dolor in hendrerit

Ullamcorper suscipit lobortis nisl ut aliquip

Duis autem vel eum irem dolor in hendrerit

Step 2

Step 1

Lorem ipsum dolor sit amet

Process

+ info

Comparison

Lorem ipsum dolor sit amet

Lorem ipsum

+ info

Lorem ipsum dolor sit amet

Timeline

2030