DataBase of HABs - Greece

Lake Kastoria Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Sep 2016Water (extra-cellular) and cyanobacterial cells (intra-cellular)-Extra-cellular ATX-a = 0.058 μg/L dmMC-RR = 0.99 μg/L MC-RR = 36.5 μg/L MC-YR = 4.65 μg/L dmMC-LR = 0.076 μg/L MC-LR = 13.2 μg/LMC-HilR = 1.07 μg/L Intra-cellular dmMC-RR = 1.99 μg/L MC-RR = 75.4 μg/L MC-YR = 11.3 μg/L dmMC-LR = 0.800 μg/L MC-LR = 39.1 μg/LMC-HilR = 1.87 μg/LMC-WR = 1.07 μg/LMC-LA < LOQMC-LW <LOQLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY & MC-LF), NOD, STX, neoSTXhttps://doi.org/10.1038/s41598-018-35428-xOct 2015Water (extra-cellular) and cyanobacterial cells (intra-cellular)-Extra-cellular dmMC-RR = 1.71 μg/L MC-RR = 62.9 μg/L MC-YR = 3.60 μg/L dmMC-LR = 0.034 μg/L MC-LR = 18.3 μg/LMC-HilR = 0.101 μg/L MC-WR = 0.507 μg/LMC-LA = 0.537 μg/LIntra-cellular dmMC-RR = 1.66 μg/L MC-RR = 73.8 μg/L MC-YR = 12.8 μg/L dmMC-LR = 1.34 μg/L MC-LR = 38.2 μg/LMC-HilR = 1.02 μg/LMC-WR = 0.930 μg/LMC-LA = 0.130 μg/L MC-LY = 0.341 μg/LMC-LW <LOQMC-LF <LOQLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-x5 Oct 2015Filtered water (extra-cellular)-Extra-cellular [D-Asp3] MC-RR = 1.7 μg/L MC-RR = 63 μg/L MC-YR = 3.6 μg/L [D-Asp3] MC-LR = 0.034 μg/L MC-LR = 18 μg/LMC-HilR = 0.10 μg/LMC-WR = 0.51 μg/LMC-LA = 0.54 μg/L SPE, LC-MS/MStargeting CYN, ATX-a, NOD, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), Domoic acid and Okadaic acidhttps://doi.org/10.1016/j.jhazmat.2016.07.020Sep 2015Filtered water (extra-cellular)-Extra-cellular dmMC-RR = 0.007 μg/L MC-RR = 0.308 μg/L MC-YR = 0.074 μg/L dmMC-LR = 0.026 μg/L MC-LR = 0.373 μg/LMC-HilR = 0.019 μg/LMC-LY = 0.018 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xAug 2015Filtered water (extra-cellular)-Extra-cellular MC-RR = 0.092 μg/L MC-YR = 0.013 μg/L MC-LR = 0.072 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xOct 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Μicrocystis aeruginosa, Microcystis wesenbergii, Microcystis panniformis,Anabaena spp.Extra-cellular STX = 52.4 μg/L dmMC-RR = 28.2 μg/L MC-RR = 338 μg/L MC-YR = 80.7 μg/L dmMC-LR = 9.60 μg/L MC-LR = 354 μg/LMC-HilR = 16.1 μg/L Intra-cellular dmMC-RR = 2.09 μg/L MC-RR = 63.2 μg/L MC-YR = 9.32 μg/L MC-HtyR = 0.294 μg/L dmMC-LR = 1.35 μg/L MC-LR = 36.8 μg/LMC-HilR = 1.57 μg/LMC-WR = 1.12 μg/LMC-LA < LOQMC-LY = 0.873 μg/LMC-LW = 0.277 μg/LMC-LF = 0.250 μg/LLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSep 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Μicrocystis aeruginosa, Anabaena cf. flos-aquae, Anabaena cf. circinalis, Microcystis panniformisExtra-cellular MC-RR = 12.3 μg/L MC-LR = 14.8 μg/LIntra-cellular STX = 0.140 μg/L dmMC-RR = 3.40 μg/L MC-RR = 43.0 μg/L MC-YR = 7.15 μg/L dmMC-LR = 2.68 μg/L MC-LR = 45.8 μg/LMC-HilR = 1.33 μg/LMC-WR = 1.27 μg/LMC-LA < LOQMC-LY = 0.746 μg/LMC-LW = 0.294 μg/LMC-LF <LOQLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-x18 Sep 2014Filtered water (extra-cellular)-Extra-cellular MC-RR =7.1 μg/L MC-YR = 1.5 μg/L [D-Asp3] MC-LR = 0.48 μg/L MC-LR = 9.0 μg/LMC-HilR = 0.42 μg/LMC-WR = 0.11 μg/LMC-LY = 0.16 μg/L SPE, LC-MS/MStargeting CYN, ATX-a, NOD, 12 MCs Domoic acid and Okadaic acidhttps://doi.org/10.1016/j.jhazmat.2016.07.02024 Aug 2010Cyanobacterial cells(intra-cellular)Microcystis wesenbergii, Planktolyngbya cicrumcreta, Microcystis novacekiiMCs were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.010Sep 2007Cyanobacterial cells(intra-cellular)Cylindrospermopsis raciborskii, Microcystis aeruginosa, Anabaena cf.flos-aquae, Microcystis panniformisIntra-cellular STX = 0.100 μg/L dmMC-RR <LOQ MC-RR = 0.403 μg/L MC-YR = 0.045 μg/LMC-LR = 0.219 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-x2008Filtered water(extra-cellular) -Etxra-cellular MC-RR = 0.008 μg/L MC-LR = 0.007 μg/LSPE, LC-MS/MS targetingMC-LR, MC -RR, MC -YR, MC -LA and NOD https://doi.org/10.1016/j.toxicon.2009.07.012Spring 2005Water and cyanobacterial cells-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.2051313 Aug 2003Water with cyanobacterial bloomCylindrospermopsis raciborskii, Microcystis aeruginosa,Limnothrix redekei,Chroococcus limneticus, Oscillatoria sp., Anabaena aphanizomenoides--https://doi.org/10.1016/j.limno.2010.10.0031 Oct 2000Cyanobacterial cells(intra-cellular) -Intra-cellularMC-LR = 2.95 μg/LMC-RR = 4.01 μg/LUnidentified MC = 0.99 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md1310631920 Sep 2000Cyanobacterial cells(intra-cellular)-Intra-cellularMC-LR = 2.40 - 5.31 μg/LMC-RR = 4.22 - 8.20 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md1310631920 Sep 2000Cyanobacterial bloomMicrocystis aeruginosa, M. flos-aquaeTotal MCs = 1157.2 μg/g (28.8% MC-LR, 56.9% MC-RR, 7.9% [Dha7] MC-RR and 6.3% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010517 Jul 2000Cyanobacterial cells(intra-cellular)-Intra-cellular MC-RR = 1.80 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063193 Jul 2000Cyanobacterial bloomMicrocystis aeruginosa, M. novacekiiTotal MCs = 1191.2 μg/g (31.3% MC-LR, 67.2% MC-RR, and 1.6% MC-LA)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010510 Oct 1999Cyanobacterial bloomMicrocystis aeruginosaTotal MCs = 2564.3 μg/g (30.6% MC-LR, 61.7% MC-RR and 7.7% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010526 Jul 1999Cyanobacterial cells(intra-cellular)-Intra-cellular MC-LR = 0.65 μg/LMC-RR = 1.96 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md13106319Mar - Oct 1999Composite water made by mixing samples from the whole water column from six stationsLimnothrix redekei, Cylindrospermopsis raciborskii, Aphanizomenon spp., MicrocystisaeruginosaTotal MCs = not detected - 34.0 μg MC-LR equivalents/LELISA (Envirologix MicrocystinPlate Kit)https://doi.org/10.1007/s10750-006-0360-4Nov 1998 - Oct 1999Water samples from surface and bottom layerLimnothrix redekei, Microcystisaeruginosa, Cylindrospermopsis raciborskii, Aphanizomenon gracile --https://doi.org/10.1007/s10750-006-0360-430 Oct 1996Cyanobacterial cells / bloom(intra-cellular)-Intra-cellular MC-LR = 1390 - 3627 μg/LMC-RR = 825 - 2473 μg/LMC-YR = 443 - 793 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md1310631927 Aug 1996Cyanobacterial cells / bloom(intra-cellular)-Intra-cellular MC-LR = 2860 μg/LMC-RR = 5970 μg/LMC-YR = 864 μg/L HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063191 Aug 1996Cyanobacterial cells(intra-cellular)-Intra-cellular MC-LR = 1.39 - 13.93 μg/LMC-RR = 3.57 - 30.55 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md1310631918 Jun 1996Cyanobacterial cells(intra-cellular)-Intra-cellular MC-LR = 2229 μg/LMC-RR = 4230 μg/LMC-YR = 635 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063195 Oct 1995Cyanobacterial bloomMicrocystis aeruginosa, M. novacekiiTotal MCs = 382.1 μg/g (27.9% MC-LR, 57.6% MC-RR, 3.6% MC-YR and 10.9% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010520 Aug 1995Cyanobacterial bloom-Total MCs = 229.5 μg/g (34.3% MC-LR, 24.3% MC-RR, 41.4% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010529 Sep 1994Cyanobacterial bloomMicrocystis aeruginosa, M. novacekii Total MCs = 1040.9 μg/g (30.6% MC-LR, 3.0% D-Asp3] or [Dha7] MC-LR, 50.8% MC-RR, 2.4% [Dha7] MC-RR, 7.0% MC-YR and 6.3% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201058 Sep 1994Cyanobacterial bloomMicrocystis aeruginosa, M. novacekiiTotal MCs = 556.4 μg/g (29.3% MC-LR, 46.1% MC-RR, 2.5% [Dha7] MC-RR, 6.0% MC-YR and 16.2% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010520 Aug 1994Cyanobacterial bloomMicrocystis aeruginosaTotal MCs =1178.8 μg/g (24.6% MC-LR, 51.0% MC-RR, 5.7% MC-YR and 18.8% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201054 Aug 1994 (1) Cyanobacterial bloomCylindrospermopsis raciborskii, Limnothrix redekeiTotal MCs = 1168.5 μg/g (24.5% MC-LR, 57.6% MC-RR, 5.6% MC-YR and 11.4% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201054 Aug 1994 (2)Cyanobacterial bloomMicrocystis aeruginosa, Limnothrix redekeiTotal MCs = 114.5 μg/g (25.2% MC-LR, 68.1% MC-RR, and 6.7% MC-YR)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010523 Jul 1994Cyanobacterial bloomMicrocystis novacekii, M.aeruginosa Total MCs = 1305.7 μg/g (27.3% MC-LR, 50.3% MC-RR, 7.9% MC-YR and 14.6% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010530 May 1994Cyanobacterial bloomMicrocystis aeruginosa, M. novacekiiTotal MCs = 221.9 μg/g (36.0% MC-LR, 39.0% MC-RR, 11.8% MC-YR and 13.2% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.20105-Cyanobacterial bloomMicrocystis, Anabaena, CylindrospermopsisLD50 = 40 - 1500 mg dw/kgTotal MCs = 1638 ± 464 μg/g dwMice bioassay – LD50 intraperitoneal injection & HPLC-UV https://doi.org/10.1002/aheh.20030052326 Oct 1987Surface water containing bloomMicrocystis aeruginosaLD50 = 300 - 600 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1007/BF0000353717 Sep 1987Surface water containing bloomMicrocystis aeruginosa LD50 = 60 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1007/BF000035377 Sep 1987Surface water containing bloomMicrocystis aeruginosa, Anabaena viguieriLD50 = 125 - 550 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1007/BF0000353721 Aug 1987Surface water containing bloomAnabaena viguieri, Microcystis aeruginosaLD50 = 40 - 1500 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1007/BF00003537

Lake Volvi Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference 16 May 2011Cyanobacterial cellsAnabaena perturbata MCs were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.01021 Aug 2010Cyanobacterial cellsCylindrospermopsis raciborskiiMCs and CYN were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.010July 2009Cyanobacterial cells(Intra-cellular)Cylindrospermopsis raciborskii, Anabaena aphanizomenoides, Anabaenopsis elenkiniiIntra-cellularMC-RR < LOQLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005Water and cyanobacterial cells (scum)-Water:Total MCs = 209.56 ng/LCyanobacterial cells:Total MCs = 1086 ng/LELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.205131994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, Anabaenopsis cunningtonii, Microcystis novacekii, Anabaena circinalis, Cylindrospermopsis raciborskii--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomAphanocapsa, Microcystis, Anabaena, Anabaenopsis, Aphanizomenon, CylindrospermopsisLD50 = 1500 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1002/aheh.2003005239 Aug 1987Surface water containing bloom-LD50 = 1500 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1007/BF00003537

Lake Koronia Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Spring 2005Water (extra-cellular) and cyanobacterial cells (intra-cellular)-Extra-cellularTotal MCs = 3748.6 ng/LIntra-cellularTotal MCs = 15,896 ng/LELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.20513Aug - Sep 2004Cyanobacterial bloomAnabaena aphanizomenoides, Anabaena sp., Anabaenopsis elenkinii, Merismopedia minima, Planktothrix sp.--https://doi.org/10.1093/plankt/fbn114-Cyanobacterial bloomAnabaenopsisLD50 = 600 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1002/aheh.20030052328 Sep 1987Surface water containing bloomAnabaenopsis milleriLD50 = 600 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1007/BF00003537

Lake Vistonis (or Porto-Lagos) Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference 21 Aug 2014Cyanobacterial cells (intra-cellular)Aphanizomenon favaloroi, Pseudanabaena limnetica,Limnothrix sp., Merismopedia punctata,unidentified dinophyte, Scenedesmus spp., Monoraphidium griffithii, Nitzschia acicularisIntra-cellularSTX = 42 μg/g dw neo STX = 17 μg/g dwLC-MS/MS targeting CYN, ATX-a, 12 MCs, STX, neo-STX and NODhttps://doi.org/10.1071/MF160295 Aug 2014Cyanobacterial cells (intra-cellular)Aphanizomenon favaloroiIntra-cellularSTX = 0.4 μg/g dwneo STX = 1.6 μg/g dwLC-MS/MS targeting CYN, ATX-a, 12 MCs, STX, neo-STX and NODhttps://doi.org/10.1071/MF16029Aug 2014Cyanobacterial cells (intra-cellular)Aphanizomenon favaloroiIntra-cellularSTX = 0.040 μg/L neo STX = 0.160 μg/LLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xJuly 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Aphanizomenon favaloroiExtra-cellularnot detectedIntra-cellular STX = 4.20 μg/L neo STX = 1.70 μg/LLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NOD https://doi.org/10.1038/s41598-018-35428-x2 Aug 1999Cyanobacterial bloomMicrocystis aeruginosa, Microcystis spp.Total MCs = 317.2 μg/g (100% MC-LR) HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010512 Jul 2000Cyanobacterial cells(intra-cellular)-Unidentified MC = 0.46 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063193 Aug 1999 Cyanobacterial cells(intra-cellular) -MC-RR = 0.90 μg/L Unidentified MC = 0.42 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063192 Aug 1999Cyanobacterial cells(intra-cellular) -Unidentified MCs = 0.88 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063191994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, M. ichthyoblabe, M. wesenbergii, Anabaena sp.--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomMicrocystis, Oscillatoria, Anabaena, AnabaenopsisLD50 = 1130 - 1500 mg dw/kgTotal MCs = 317.2 μg/g dwMice bioassay – LD50 intraperitoneal injection & HPLC-UVhttps://doi.org/10.1002/aheh.200300523-Cyanobacterial bloomAnabaenopsis milleri WoronichinLD50 = 600 - 1500 mg dw/kgprobably MC-LRMice bioassay – LD50 intraperitoneal injection TLC, HPTLC & HPLC-UVhttps://doi.org/10.1016/0048-9697(94)90324-718 Aug 1987Surface water containing bloomAnabaenopsis milleri, Microcystis aeruginosa, Oscillatoria sp.LD50 = 1500 mg dw/kgMice bioassay – LD50 intraperitoneal injection https://doi.org/10.1007/BF00003537

Lake Kerkini Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Oct 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Cylindrospermopsis raciborskii, Planktothrix agardhii, Anabaenopsis elenkiniiExtra-cellular not detected Intra-cellular STX = 6.65 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-x21 Aug 2010Cyanobacterial cellsCylindrospermopsis raciborskiiMCs and STXs were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.010June 2008Cyanobacterial cells (intra-cellular)Aphanizomenon flos-aquae, Microcystis aeruginosa, Anabaena cf. viguieri, Anabaenopsis elenkinii, Anabaena flos-aquae, Microcystis panniformis, Pseudanabaena limnetica, AnabaenaaphanizomenoidesIntra-cellular STX = 1.48 μg/L neoSTX = 0.587 μg/L ATX-a = 0.607 μg/L MC-RR = 0.034 μg/L MC-LR = 0.137 μg/L MC-LW < LOQLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.2051326 Aug 1999Cyanobacterial bloomMicrocystis aeruginosa, Anabaena spiroidesTotal MCs = 43.9 μg/g (33.6% MC-LR, 66.4% MC-RR) HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201053 Aug 1999Cyanobacterial cells(intra-cellular)-MC-LR = 4.21 μg/LMC-RR = 5.27 μg/LUnidentified MC = 2.26 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063193 Aug 1999Cyanobacterial bloomMicrocystis spp., M. wesenbergiiTotal MCs = 93.5 μg/g (56.1% MC-LR, 34.1% MC-RR, 4.9% [Dha7]MC-RR, 4.9% MC-YR) HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010511 Aug 1994 (2)Cyanobacterial bloomMicrocystis aeruginosaTotal MCs = 87.4 μg/g (12.5% MC-LR, 15.2% MC-RR, 4.1% MC-YR, 68.2% unidentified MCs) HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010511 Aug 1994 (1)Cyanobacterial bloomMicrocystis aeruginosa, M. flos-aquaeTotal MCs = 598.6 μg/g (30.1% MC-LR, 50.8% MC-RR, 5.0% MC-YR, 14.1% unidentified MCs) HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201051994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, M. flos-aquae, M. ichthyoblabe, M. novacekii, M. wesenbergii, Pseudanabaena sp., Anabaena flos-aquae, Anabaena viguieri, A. spiroides, A. issatschenkoi--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomMicrocystis, Anabaena, AphanizomenonTotal MCs = 68.7 ± 24.8 μg/g dw HPLC-UV https://doi.org/10.1002/aheh.200300523

Lake Karla Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Jul 2013 - Jul 2015 Filter extracts (intra-cellular)Anabaenopsis elenkinii, Sphaerospermopsis aphanizomenoides, Limnothrix redekei, Planktothrix cf. agardhii, Cylindrospermopsisraciborskii, Microcystis aeruginosa,Dolichospermum (Anabaena) cf. smithiiIntra-cellular STX : <0.180 μg/L neo-STX: <0.300 μg/L CYN: 0.5 - 12.1 μg/L ATX-a: 0.8 - 5.4 μg/L dmMC-RR: <0.126 μg/L MC-RR: <0.054 μg/L MC-YR:<0.264 μg/L MC-LR: <0.258 μg/LLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.4081/aiol.2017.635020 Apr 2011Cyanobacterial cellsPlanktothrix agardhii, Limnothrix redekei, Anabaenopsis elenkinii, Merismopedia tenuissima, Aphanizomenon cf. floa-aquae, Anabaena spiroides MCs, STXs and CYN were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.01026 Mar 2010Cyanobacterial cellsLimnothrix redekei, Planktothrix agardhii, Anabaenopsis elenkiniinot detected ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.0109 Aug 2010Cyanobacterial cellsMicrocystis spp., Planktothrix agardhii MCs and CYN were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.010June 2011Water (extra-cellular) and cyanobacterial cells (intra-cellular)-Extra-cellular Total MCs = 3.01 μg/L Intra-cellular Total MCs = 5.50 μg/LELISA(Abraxis Microcystins kit)https://doi.org/10.4194/1303-2712-v14_1_23May 2011Water (extra-cellular) and cyanobacterial cells (intra-cellular)Anabaenopsis sp., PlanktothrixExtra-cellular Total MCs = 2.03 μg/L Intra-cellular Total MCs = 4.19 μg/LELISA(Abraxis Microcystins kit)https://doi.org/10.4194/1303-2712-v14_1_23April - October 2010Water (extra-cellular) and cyanobacterial cells (intra-cellular)Anabaenopsis elenkinii, Sphaerospermopsisaphanizomenoides, Planktothrix agardhiiExtra-cellular Total MCs = 0.75 - 3.90 μg/L Intra-cellular Total MCs = 1.01 - 9.83 μg/LELISAhttps://doi.org/10.1016/j.scitotenv.2013.02.03520 Apr 2010Water with cyanobacterial bloomAnabaenopsis Elenkinii, Planktothrix cf. agardhii --https://doi.org/10.1100/2012/50413517 Mar 2010Water with cyanobacterial bloomPlanktothrix cf. agardhii, Anabaena sp.--https://doi.org/10.1100/2012/504135

Lake Doirani Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference 21 Aug 2010Cyanobacterial cellsMicrocystis wesenbergii, Aphanizomenon issatschenkoi, Planktolyngbya circumcretaMCs were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.01029 Sep 2009Cyanobacterial cells Microcystis wesenbergii, Microcystis aeruginosa, Aphanizomenon cf. flos-aquaeMCs and STXs were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.010Sep 2008Cyanobacterial cells(intra-cellular)Cylindrospermopsis raciborskii/Raphidiopsis mediterranea, Microcystis aeruginosa, Aphanizomenon issatschenkoi, Aphanizomenongracile, Anabaena flos-aquae, Anabaena aphanizomenoidesIntra-cellular CYN = 0.024 μg/L ATX-a = 0.926 μg/L dmMC-RR <LOQ MC-RR = 0.363 μg/L MC-YR = 0.070 μg/L MC-LR = 0.171 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005 Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.2051312 Aug 2004Water with cyanobacterial bloomCylindrospermopsis raciborskii, Aphanizomenon issatshenkoi, Planktothrix cf. agardhii, Snowella lacustris, Anabaena flos-aquae, Jaaginema sp., Microcystis flos-aquae, Aphanocapsa incerta, Microcystis aeruginosa, Microcystis flos-aquae, Snowella lacustris, Synechococcus spp., Planktolyngbya circumcreta--https://doi.org/10.1016/j.limno.2010.10.0033 Aug 1999Cyanobacterial cells(intra-cellular)-MC-YR = 4.42 μg/LUnidentified MC = 10.03 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063191994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, Jaaginema subtilissimum, Anabaena aphanizomenoides, Anabaena flos-aquae, Aphanizomenon flos-aquae--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomMicrocystis, Anabaena, Anabaenopsis, Aphanizomenon- https://doi.org/10.1002/aheh.200300523

Lake Agra Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Cyanobacterial bloomAphanocapsa, Anabaena- https://doi.org/10.1002/aheh.200300523

Lake Vegoritis Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference May - Oct 2020Water (extra-cellular) and cyanobacterial cells (intra-cellular)Dolichospermum spp., Aphanocapsa cf. holsatica, Aphanizomenon spp., Microcystis spp.Extra-cellular CYN = 0.007 - 0.112 μg/L MC-RR = n.d. - 0.021 μg/L MC-YR = n.d. - 0.155 μg/L MC-HtyR = n.d. - 0.069 μg/L dmMC-LR = n.d. - 0.036 μg/L MC-LR = n.d. - 0.315 μg/LMC-HilR = n.d. - 0.033 μg/L Intra-cellular CYN = n.d. - 0.075 μg/L dmMC-RR = n.d. - 0.010 μg/L MC-RR = n.d. - 0.674 μg/L MC-YR = n.d. - 0.199 μg/L MC-HtyR = n.d. - <LOQ dmMC-LR = n.d. - 0.020 μg/L MC-LR = n.d. - 0.146 μg/LMC-HilR = n.d. <LOQSee figure 1 below LC-MS/MStargeting CYN, ATX-a, NOD, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF)https://doi.org/10.3390/toxins13060394Apr - Oct 2019Water (extra-cellular) and cyanobacterial cells (intra-cellular)Aphanizomenon spp.,Lemmermanniella spp., Raphidiopsis raciborskii and Dolichospermum spp.Extra-cellular CYN = n.d. - 0.114 μg/L dmMC-LR = n.d. - 0.080 μg/L MC-LR = n.d. - 0.233 μg/L Intra-cellular CYN = n.d. - 0.685 μg/L MC-RR = n.d. - 0.029 μg/L MC-HtyR = n.d. - <LOQ dmMC-LR = n.d. - 0.055 μg/L MC-LR = n.d. - 0.241 μg/LMC-HilR = n.d. - 0.027 μg/L See figure 1 below LC-MS/MStargetingCYN, ATX-a, NOD,12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF)https://doi.org/10.3390/toxins13060394Jul - Nov 2018Water (extra-cellular) and cyanobacterial cells (intra-cellular)Microcystis spp., Aphanocapsa spp., Dolichospermum spp. and Aphanizomenon flos-aquaeExtra-cellular MC-RR = n.d. - 0.023 μg/L MC-YR = n.d. - 0.014 μg/L MC-LR = n.d. - 0.029 μg/L Intra-cellular dmMC-RR = n.d. - 0.003 μg/L MC-RR = n.d. - 0.074 μg/L MC-YR = n.d. - 0.026 μg/L dmMC-LR = n.d. - <LOQ MC-LR = n.d. - 0.055 μg/LSee figure 1 below LC-MS/MStargetingCYN, ATX-a, NOD,12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF)https://doi.org/10.3390/toxins13060394July 2014Filtered water (extra-cellular)-Extra-cellular dmMC-RR < LOQ MC-RR = 104 μg/L MC-YR = 29.3 μg/L dmMC-LR = 10.9 μg/L MC-LR = 96.3 μg/LMC-LY = 18.1 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSep 2008Cyanobacterial cells(intra-cellular)Anabaena viguieri, Aphanizomenon flos-aquae, Anabaena flos-aquae, Microcystis aeruginosaIntra-cellular CYN < LOQ MC-RR = 0.118 μg/L MC-LR = 0.049 μg/LLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.20513-Cyanobacterial bloomAnabaena, Aphanizomenon- https://doi.org/10.1002/aheh.200300523* n.d. : not detected Figure 1: Occurence of cyanotoxins in Lake Vegoritis during the sampling periods 2018, 2019 and 2020 at (a) sampling site 1 and (b) sampling site 2. Reprinted from https://doi.org/10.3390/toxins13060394 (CC BY)

Lake Petron Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Sep 2008Cyanobacterial cells(intra-cellular)Cylindrospermopsis raciborskii, Cyanodictyon imperfectum, Planktolyngbya limnetica, Aphanizomenon gracile, Anabaenopsis elenkinii, Planktolyngbya microspira,Planktothrix agardhiiIntra-cellular STX = 0.159 μg/L MC-RR = 0.003 μg/L MC-LR = 0.006 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA (Abraxis Microcystins Kit)https://doi.org/10.1002/tox.20513-Cyanobacterial bloomMicrocystis, Aphanizomenon-https://doi.org/10.1002/aheh.200300523

Lake Mikri (or Lesser) Prespa Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference 18 Sep 2015Cyanobacterial cells - filter(intra-cellular)-Intra-cellularMC-YR = 0.031 μg/LMC-RR = 0.228 μg/LdmMC-LR = 0.008 μg/LMC-LR = 0.128 μg/L LC-MS/MS targetingMC-YR, dmMC-RR, MC-RR, dmMC-LR, MC-LR,MC-LY, MC-LW, MC-LF, NOD, CYN, ATX-ahttps://doi.org/10.3390/toxins10040156Nov 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Microcystis aeruginosaExtra-cellular MC-RR = 41.4 μg/L MC-YR = 36.2 μg/L MC-LR = 40.8 μg/L Intra-cellular MC-RR = 0.354 μg/L MC-YR <LOQ dmMC-LR <LOQ MC-LR <LOQLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-x6 Nov 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 0.17 - 0.30 μg/L MC-RR = 14.38 - 28.56 μg/L MC-YR = 34.83 - 67.35 μg/L dmMC-LR = 1.32 - 2.02 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w1303035730 Oct 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 0.58 - 13.70 μg/L MC-RR = 119.30 - 601.45 μg/L MC-YR = 49.10 - 140.85 μg/L dmMC-LR = 3.08 - 6.68 μg/L MC-LR = 24.81 - 97.72 μg/L MC-LY = n.d. - 0.92 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w1303035721 Oct 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 19.42 - 19.73 μg/L MC-RR = 616.80 - 635.83 μg/L MC-YR = 18.38 - 67.50 μg/L dmMC-LR = 4.85 - 5.65 μg/L MC-LR = n.d. - 131.75 μg/L MC-LY = n.d. - 1.28 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w1303035720 Sep 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 0.26 - 0.94 μg/L MC-RR = 60.41 - 151.78 μg/L MC-YR = 16.89 - 40.45 μg/L dmMC-LR = 1.33 - 1.71 μg/L MC-LR = 13.36 - 37.47 μg/L MC-LY = n.d. - 0.14 μg/L MC-LF = n.d. - 0.11 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w1303035712 Sep 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 1.31 - 1.64 μg/L MC-RR = 242.02 - 246.13 μg/L MC-YR = 67.33 - 69.47 μg/L dmMC-LR = 3.02 - 3.21 μg/L MC-LR = 66.29 - 69.32 μg/L LY = 0.34 - 0.35 μg/L MC-LF = 0.25 - 0.35 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w130303578 Sep 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 1.10 μg/L MC-RR = 167.24 μg/L MC-YR = 46.19 μg/L dmMC-LR = 2.35 μg/L MC-LR = 48.77 μg/L MC-LY = 0.12 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w130303574 Sep 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 0.02 - 0.39 μg/L MC-RR = 16.20 - 203.76 μg/L MC-YR = 2.11 - 18.87 μg/L dmMC-LR = 0.03 - 0.82 μg/L MC-LR = 6.36 - 76.91 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w130303574 Aug 2014Cyanobacterial cells - filter(intra-cellular)-Intra-cellular dmMC-RR = 0.61 μg/L MC-RR = 93.66 μg/L MC-YR = 32.42 μg/L dmMC-LR = 0.96 μg/L MC-LR = 31.46 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w130303573 Sep 2013Cyanobacterial cells - filter and seston(intra-cellular) Microcystis wesenbergii and M. aeruginosaIntra-cellular dmMC-RR = n.d. - 0.03 μg/L MC-RR = 0.14 - 0.58 μg/L MC-YR = 0.08 - 0.36 μg/L dmMC-LR = 0.01 - 0.02 μg/L MC-LR = 0.12 - 0.57 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NOD, STX, dcSTX, NEO, dcNEO, GTX1, GTX2, GTX3, GTX4, dcGTX2, dcGTX3, ATX-a, HATX-a, dihydroATX-A, dihydroHATX-a, epoxyATX-a, epoxyHTAX-a, CYN, 7-epi-CYN, 7-deoxy-CYNhttps://doi.org/10.3390/w1303035731 Aug 2012Cyanobacterial cells - filter(intra-cellular)Microcystis wesenbergii, Dolichospermum lemmermannii, M. aeruginosaIntra-cellular dmMC-RR = n.d. - 2.43 μg/L MC-RR = n.d. - 35.40 μg/L MC-YR = n.d. - 4.77 μg/L dmMC-LR = n.d. - 0.44 μg/L MC-LR = 0.04 - 14.81 μg/L MC-LY = n.d. - 0.25 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w1303035714 Aug 2012Cyanobacterial cells - filter(intra-cellular)Microcystis wesenbergii, Dolichospermum lemmermannii, M. aeruginosaIntra-cellular MC-RR = n.d. - 0.22 μg/L MC-YR = n.d.- 0.02 μg/L MC-LR = 0.03 - 0.06 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w130303573 Aug 2012Cyanobacterial cells - filter(intra-cellular)Microcystis wesenbergii, Dolichospermum lemmermannii, M. aeruginosaIntra-cellular dmMC-RR = 0.26 - 0.28 μg/L MC-RR = 1.19 - 1.52 μg/L MC-YR = 0.09 - 0.12 μg/L MC-LR = 0.25 - 0.31 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW and MC-LF, NODhttps://doi.org/10.3390/w130303572 Aug 2012Cyanobacterial cells - filter(intra-cellular)Microcystis wesenbergii, Dolichospermum lemmermannii, M. aeruginosaIntra-cellular MC-RR = n.d. - 0.24 μg/L MC-YR = n.d. - 0.04 μg/L MC-LR = n.d. - 0.05 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW, MC-LF, NODhttps://doi.org/10.3390/w1303035719 July 2012Cyanobacterial cells - filter(intra-cellular)Microcystis wesenbergii, Dolichospermum lemmermannii, M. aeruginosaIntra-cellular dmMC-RR = n.d. - 0.40 μg/L MC-YR = n.d. - 0.04 μg/L MC-LR = n.d. - 0.04 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW, MC-LF, NODhttps://doi.org/10.3390/w130303573 July 2012Cyanobacterial cells - filter(intra-cellular)Microcystis wesenbergii, Dolichospermum lemmermannii, M. aeruginosaIntra-cellular dmMC-RR = n.d. - 0.23 μg/L LC-MS/MS targetingdm-7-MC-RR, MCRR,MC-YR, dm-7-MC-LR, MC-LR, MC-LY, MC-LW, MC-LF, NODhttps://doi.org/10.3390/w13030357Spring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit) https://doi.org/10.1002/tox.20513 5 Aug 1999Cyanobacterial cells(intra-cellular)-Intra-cellularMC-LR = 25.00 μg/LMC-RR = 64.33 μg/LMC-YR =12.08 μg/LUnidentified MC = 6.00 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063195 Aug 1999Cyanobacterial bloomMicrocystis spp., M. wesenbergiiTotal MCs = 1153.3 μg/g (25.7% MC-LR, 2.6% [D-Asp3] or [Dha7]MC-LR, 65.8% MC-RR, 0.6% MC-LA and 5.4% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201051994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, Microcystis wesenbergii, Anabaena lemmermannii--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomAphanocapsa, Microcystis, Oscillatoria, AnabaenaTotal MCs = 1091 μg/g dw HPLC-UV https://doi.org/10.1002/aheh.200300523 * n.d.: not detected

Lake Zazari Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference June 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Microcystis aeruginosa, Anabaena spiroidesExtra-cellular MC-RR = 17.5 μg/LMC-LR = 25.0 μg/L Intra-cellular MC-RR = 1.05 μg/L MC-YR = 0.819 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.205135 Aug 1999Cyanobacterial cells(intra-cellular)-MC-LR = 2.20 μg/LMC-RR = 4.35 μg/LUnidentified MC = 2.13 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063195 Aug 1999Cyanobacterial bloomMicrocystis aeruginosa, Microcystis spp. Total MCs = 50.3 μg/g (46.7% MC-LR, 53.3% MC-RR)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201051994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, Microcystis flos-aquae, Microcystis ichthyoblabe, Microcystis novacekii, Microcystis viridis, Microcystis wesenbergii, Pseudanabaena sp., Anabaena flos-aquae, Anabaena viguieri, Anabaena spiroides, Aphanizomenon issatschenkoi, Cylindrospermopsis raciborskii--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomMicrocystis, Anabaena, Aphanizomenon, CylindrospermopsisTotal MCs = 50.3 μg/g dw HPLC-UV https://doi.org/10.1002/aheh.200300523

Lake Cheimaditis Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Sep 2008Cyanobacterial cells(intra-cellular)Microcystis panniformis, Microcystis aeruginosa, Microcystis wesenbergii,Microcystis flos-aquae, Cylindrospermopsisraciborskii, Anabaena flos-aquaeIntra-cellular dmMC-RR = 0.076 μg/L MC-RR = 1.16 μg/L MC-YR <LOQ MC-LR = 0.198 μg/L MC-HilR <LOQ LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.20513-Cyanobacterial bloomMicrocystis, Oscillatoria, Anabaena- https://doi.org/10.1002/aheh.200300523

Lake Asomaton or Sfikia Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Cyanobacterial bloomOscillatoria, Aphanizomenon, Cylindrospermopsis--https://doi.org/10.1002/aheh.200300523

Lake Polyphyton Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Aug - Nov 2003Air and integrated water sampleAnabaena aphanizomenoides, Anabaena sp., Aphanizomenon issatschenkoi, Aphanizomenon ovalisporum, Aphanocapsa sp., Aphanothece sp., Chroococcus disperses, Chroococcus limneticus, Chroococcus minutus, Coelosphaerium sp., Gomphosphaeria naegeliana, Jaaginema sp., Limnothrix redekei, Merismopedia elegans, Merismopedia punctata, Microcystis sp., M. aeruginosa, Oscillatoria sp., Pannus sp., Phormidium sp., Planktolyngbya limnetica, Planktothrix sp., Pseudanabaena cf. constricta, Pseudanabaena cf. limnetica, Radiocystis geminata and Spirulina sp. --https://doi.org/10.1093/plankt/fbp038-Cyanobacterial bloomMicrocystis, Anabaena, Aphanizomenon, Cylindrospermopsis--https://doi.org/10.1002/aheh.200300523

Lake Pamvotis Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Oct 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Microcystis aeruginosa, Microcystis wesenbergii, Anabaena spp.Extra-cellular dmMC-RR <LOQ MC-RR = 66.9 μg/L MC-YR = 21.1 μg/L MC-LR = 131 μg/LIntra-cellular MC-RR = 0.450 μg/L MC-YR <LOQ MC-LR = 0.212 μg/LLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY & MC-LF), NOD, STX, neoSTXhttps://doi.org/10.1038/s41598-018-35428-xSep 2014Water (extra-cellular) and cyanobacterial cells (intra-cellular)Microcystis aeruginosa, Anabaena flos-aquae, Microcystis panniformis, Αphanizomenon flos-aquaeExtra-cellular MC-RR = 2.8 μg/L Intra-cellular CYN <LOQ MC-RR = 0.106 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY & MC-LF), NOD, STX, neoSTXhttps://doi.org/10.1038/s41598-018-35428-x7 Oct 2010Cyanobacterial cellsMicrocystis spp., Microcystis viridis, Microcystis novacekii, Microcystis aeruginosa, Microcystis flos-aguaeMCs and CYN were detected * concentration values are given in Charts - see reference ELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.toxicon.2013.11.010January 2008–February 2009Water (extra-cellular) and cyanobacterial cells (intra-cellular)Microcystis wesenbergii, Microcystis aeruginosa, Anabaena flos-aquae, Anabaena spiroides, Anabaena viguieri,Aphanizomenon issatschenkoi,Aphanizomenon flos-aquae Extra-cellular Total MCs = <1 μg/L - 9 μg/L Intra-cellular Total MCs = <1 μg/L- 19 μg/L STXs were detected in two samples at 1.3 μg/L (Jul 2008) and 2.1 μg/L (Sep 2008) CYN was not detectedELISA(Abraxis Microcystin, Saxitoxin andCylindrospermopsin kits)https://doi.org/10.1016/j.hal.2014.09.002January - December 2008Water (extra-cellular) and cyanobacterial cells (intra-cellular)Microcystis sp. (all the period)and Anabaena sp (June and July)Extra-cellular 0.0121 - 7.88 μg MC-LR eq/L Intra-cellular 0.15 - 15.21 μg MC-LR eq/L(Maximum values during July - October)ELISA and confirmation of MC-LR by HPLC-UV https://doi.org/10.1007/s10661-011-2169-5October 2007 / June 2008 / December 2008Water (extra-cellular) and cyanobacterial cells (intra-cellular)-Extra-cellular 78 - 2500 ng MC-LR eq/L Intra-cellular 109 - 4578 ng MC-LR eq/LELISA (Abraxis Microcystins Kit)https://doi.org/10.1039/C2EM30129FSpring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA (Abraxis Microcystins Kit)https://doi.org/10.1002/tox.20513March 2005–October 2005Surface water (extra-cellular) and cyanobacterial blooms (intra-cellular)Microcystis sp, Anabaena spExtra-cellular Total MCs = 310 - 2,388 ng/L Intra-cellular Total MCs = 3,109 - 11,585 ng/L ELISA (Abraxis Microcystins Kit)https://doi.org/10.1007/s10661-007-9739-6August 2004 - December 2005WaterMicrocystis sp. and Anabaena sp./Aphanizomenon sp.Cyanobacterial pellets Total MCs = 0.01 μg/L - 19.5 μg/L ELISA (Abraxis Microcystins Kit)https://doi.org/10.1039/B903093J18 Aug 2000 (1)Cyanobacterial bloom Anabaena flos-aquae, Microcystis spp.Total MCs = 907.6 μg/g (67.1% MC-LR, 25.5% MC-RR and 7.3% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010518 Aug 2000 (2)Cyanobacterial bloomMicrocystis spp., Anabaena flos-aquaeTotal MCs = 1013.8 μg/g (77.4% MC-LR, 4.7% [D-Asp3] or [Dha7] MC-LR, 11.0% MC-RR and 6.9% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010517 Aug 2000Cyanobacterial cells(intra-cellular)-Intra-cellularMC-LR = 34.92 - 13231 μg/LMC-RR = 3.28 - 883 μg/LMC-YR = 8.20 μg/LUnidentified MC = 5.17 - 418 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063195 Aug 2000Cyanobacterial cells / bloom(intra-cellular)-Intra-cellularMC-LR = 1.08 - 9964 μg/LMC-RR = 0.66 - 169 μg/LUnidentified MC = 1.87 - 160 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063195 Aug 2000Cyanobacterial bloomMicrocystis aeruginosa, Anabaena flos-aquaeTotal MCs = 1132.0 μg/g (39.3% MC-LR, 57.9% MC-RR and 2.8% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010522 Jul 1999Cyanobacterial cells(intra-cellular)-Intra-cellularMC-LR = 7.34 μg/LMC-RR = 11.39 μg/LMC-YR = 2.02 μg/LUnidentified MC = 2.21 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md1310631922 Jul 1999Cyanobacterial bloomMicrocystis aeruginosaTotal MCs = 778.1 μg/g (41.6% MC-LR, 53.8% MC-RR and 4.6% unidentified MCs)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.201051994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, M. flos-aquae, M. novacekii, M. viridis, M. wesenbergii, Anabaena flos-aquae, Aphanizomenon flos-aquae--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomAphanocapsa, Microcystis, Oscillatoria, Anabaena, AphanizomenonTotal MCs = 958 ± 75 μg/g dw HPLC-UV https://doi.org/10.1002/aheh.200300523

Lake Kremaston Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Cyanobacterial bloomMicrocystishttps://doi.org/10.1002/aheh.200300523

Lake Voulkaria Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Cyanobacterial bloomMicrocystis, Oscillatoria, Aphanizomenon--https://doi.org/10.1002/aheh.200300523

Lake Amvrakia Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference 5 Aug 2000Cyanobacterial cells(intra-cellular)-Unidentified MC = 1.40 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md1310631918 Aug 1999 (1)Cyanobacterial bloomAnabaena perturbataTotal MCs = 42.2 μg/g (33.3% MC-LR and 66.7% MC-RR)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010518 Aug 1999 (2)Cyanobacterial bloomAnabaena perturbata, Microcystis spp.Total MCs = 43.4 μg/g (45.4% MC-LR and 54.6% MC-RR)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010510 Aug 1999 (1)Cyanobacterial bloomMicrocystis spp., Anabaena viguieriTotal MCs = 166.9 μg/g (29.4% MC-LR and 70.6% MC-RR)HPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.1002/tox.2010510 Aug 1999 (2)Cyanobacterial cells(intra-cellular)-MC-RR = 269 μg/LUnidentified MC = 523 μg/LHPLC – DAD targeting MC-LR, [D-Asp3] MC-LR, [Dha7] MC-LR, [D-Asp3, Dha7] MC-LR, MC-RR, [D-Asp3] MC-RR, [Dha7] MC-RR, [D-Asp3, Dha7] MC-RR, MC-LA, and MC-YRhttps://doi.org/10.3390/md131063191994, 1995, 1999 and 2000Surface waterMicrocystis aeruginosa, M. flos-aquae, M. novacekii, M. wesenbergii, Anabaena perturbata, A. viguieri--https://doi.org/10.1007/s10811-005-8700-8-Cyanobacterial bloomMicrocystis, AnabaenaTotal MCs = 84 ± 41 μg/g dw HPLC-UV https://doi.org/10.1002/aheh.200300523

Lake Ozeros Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Cyanobacterial bloomOscillatoria, Anabaena, Aphanizomenon--https://doi.org/10.1002/aheh.200300523

Lake Trichonis Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Nov 2018 (shallow)Cyanobacterial cells(intra-cellular)Aphanizomenon flos-aquae, Planktothrix rubescensIntra-cellular STX = 0.177 μg/L neoSTX = 0.518 μg/L dmMC-RR = 2.62 μg/L MC-YR <LOQ dmMC-LR = 0.074 μg/L LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY & MC-LF), NOD, STX, neoSTXhttps://doi.org/10.1038/s41598-018-35428-xNov 2018(deep)Cyanobacterial cells(intra-cellular)Planktothrix rubescensIntra-cellular STX = 1.59 μg/L dmMC-RR = 0.020 μg/L MC-YR <LOQ dmMC-LR <LOQ LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY & MC-LF), NOD, STX, neoSTXhttps://doi.org/10.1038/s41598-018-35428-xSpring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.2051322 Jul 1999Surface waterAphanizomenon ovalisporum, Anabaena sp.Not detectedHPLC targeting MC–LR, [D-Asp3]MC–LR, [Dha7]MC–LR, [D-Asp3,Dha7]MC–LR, MC-RR, [D-Asp3]MC-RR, [Dha7]MC-RR, [D-Asp3,Dha7] MC-RR, MC-LA and MC-YRhttps://doi.org/10.1093/plankt/fbi085-Cyanobacterial bloomOscillatoria, Anabaena, Aphanizomenon--https://doi.org/10.1002/aheh.200300523

Lake Lysimachia Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Spring 2005Water and cyanobacterial cells (scum)-MCs were detected in both sample types * MC concentration values are given in Charts - see reference ELISA(Abraxis Microcystins kit)https://doi.org/10.1002/tox.2051322 Jul 1999Surface waterAphanizomenon ovalisporum, Pseudanabaena sp., Planktothrix mougeotii MC-LR = 0.9 μg/L HPLC targeting MC–LR, [D-Asp3]MC–LR, [Dha7]MC–LR, [D-Asp3,Dha7]MC–LR, MC-RR, [D-Asp3]MC-RR, [Dha7]MC-RR, [D-Asp3,Dha7] MC-RR, MC-LA and MC-YRhttps://doi.org/10.1093/plankt/fbi08520 Jul 1999Cyanobacterial cells (intra-cellular)-MC-LR = 0.90 μg/LHPLC targeting MC–LR, [D-Asp3]MC–LR, [Dha7]MC–LR, [D-Asp3,Dha7]MC–LR, MC-RR, [D-Asp3]MC-RR, [Dha7]MC-RR, [D-Asp3,Dha7] MC-RR, MC-LA and MC-YRhttps://doi.org/10.3390/md13106319-Cyanobacterial bloomOscillatoria, Anabaena, Aphanizomenon--https://doi.org/10.1002/aheh.200300523

Lake Marathonas Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference 5 Oct 2015Filter(intra-cellular)-Intra-cellular MC-YR = 0.024 μg/LMC-RR = 0.023 μg/LdmMC-LR = 0.002 μg/LMC-LR = 0.038 μg/L LC-MS/MS targetingCYN, ATX-a, MC-YR, dmMC-RR, MC-RR, dmMC-LR, MC-LR,MC-LY, MC-LW, MC-LF, NODhttps://doi.org/10.3390/toxins100401565 Oct 2015Filtered water (extra cellular)-Extra-cellular MC-RR = 0.062 μg/L MC-YR = 0.050 μg/L [D-Asp3] MC-LR = 0.004 μg/L MC-LR = 0.063 μg/LSPE, LC-MS/MS targeting CYN, ATX-a, NOD, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), Domoic acid and Okadaic acidhttps://doi.org/10.1016/j.jhazmat.2016.07.020Sep 2013Water (extra-cellular) and cyanobacterial cells (intra-cellular)-Extra-cellular not detected Intra-cellular not detectedLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NODhttps://doi.org/10.1038/s41598-018-35428-x26 Oct 2010Water (extra-cellular) with cyanobacterial bloom (intra-cellular)-Extra-cellular MC-RR = 27μg/L MC-LR = 12 μg/L MC-YR = 26 μg/L Intra-cellular MC-RR = 1956 μg/g MC-LR = 382 μg/g MC-YR = 555 μg/gSPE, LC-MS/MS targetingMC-LR, MC -RR, MC -YR, MC -LA and NOD https://doi.org/10.1016/j.jhazmat.2013.07.036July 2007 - December 2010Filtered water (extra-cellular)-Extra-cellular MC-RR: 2 - 174 ng/L MC-LR: 2 - 451 ng/L MC-YR: 2 - 717 ng/L MC-LA: 5 - 8 ng/L See Figure 1 belowSPE, LC-MS/MS targetingMC-LR, MC -RR, MC -YR, MC -LA and NOD https://doi.org/10.1016/j.jhazmat.2013.07.036October 2007 & September 2008WaterMicrocystis aeruginosa, Chroococcus disperses, Chroococcales sp.--https://doi.org/10.1007/s10661-010-1378-7July–September 2007WaterMicrocystis aeruginosa, Anabaena bergii, Aphanizomenon sp., Aphanocapsa delicatissima, Chroococcus disperses, Chroococcus limneticus, Limnothrix redekei, Merismopedia sp., Pseudoanabaena sp.--https://doi.org/10.1007/s10661-010-1851-3-Filtered water(extra-cellular)-Extra-cellular MC-RR = 0.006 – 0.060 μg/L MC-YR = 0.000 – 0.004 μg/L MC-LR = 0.004 – 0.015 μg/LSPE, LC-MS/MS targetingMC-LR, MC -RR, MC -YR, MC -LA and NOD https://doi.org/10.1016/j.toxicon.2009.07.012-Cyanobacterial bloomMicrocystis, Cylindrospermopsis--https://doi.org/10.1002/aheh.200300523 Figure 1: Variation of MC-LR, MC-RR and MC-YR extra-cellular concentrations from July 2007 to October 2011, Lake Marathonas - Reprinted from https://doi.org/10.1016/j.jhazmat.2013.07.036 with permission from Elsevier (License Number: 5267590546619)

Lake Yliki Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Filtered water-MCs were not detectedELISAPPIA &SPE, LC-MS/MS targetingMC-LR, MC -RR, MC -YR, MC -LA and NOD https://doi.org/10.1016/j.toxicon.2009.07.012-Cyanobacterial bloomAphanocapsa, Oscillatoria, Anabaena, Anabaenopsis, Aphanizomenon, Cylindrospermopsis--https://doi.org/10.1002/aheh.200300523

Lake Stymfalia Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Cyanobacterial bloomAnabaena, Aphanizomenon--https://doi.org/10.1002/aheh.200300523

Lake Paralimni Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Cyanobacterial bloomAnabaena--https://doi.org/10.1002/aheh.200300523

Lake Mornos Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference -Filtered water-MCs were not detectedELISAPPIA &SPE, LC-MS/MS targetingMC-LR, MC -RR, MC -YR, MC -LA and NOD https://doi.org/10.1016/j.toxicon.2009.07.012-Cyanobacterial bloomAnabaena--https://doi.org/10.1002/aheh.200300523

Lake Ziros Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference January 2006 - March 2007Cyanobacterial bloomPlanktothrix rubescens, Nostoc linckia, Aphanizomenon sp, Phormidium sp, Nostoc sp, Anabaena variabilis, Phormidium uncinatum, Microcystis sp., Synechococcus sp.Cyanobacterial pelletsTotal MCs = 0 – 199 μg/LELISA (Abraxis Microcystins Kit)https://doi.org/10.1016/j.hal.2008.09.005

Lake Ismaris Time period Sample type Dominant Cyanobacteria speciesOccurrence of Cyanotoxins Analytical method Reference Aug 2011Cyanobacterial cells(intra-cellular)Anabaenopsis arnoldiiIntra-cellularnot detectedLC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NOD https://doi.org/10.1038/s41598-018-35428-xSep 2010Cyanobacterial cells(intra-cellular)Planktothrix agardhii, Anabaena cf. viguieri, Anabaena aphanizomenoides, Anabaenopsis elenkiniiIntra-cellular not detected LC-MS/MStargeting CYN, ATX-a, 12 MCs (i.e., [D-Asp3]MC-RR, MC-RR, [D-Asp3]MC-LR, MC-LR,MC-HilR, MC-YR, MC-HtyR, MC-WR, MC-LA, MC-LW, MC-LY and MC-LF), STX, neoSTX, NOD https://doi.org/10.1038/s41598-018-35428-x