EXPECTED OUTCOME
- Increase of cancer cells autophagy
- Decrease in migration of cancer cells
- 1184.08€ instead of 1319.23€
TIMING & BUDGET
- Two weeks to perform experiments
A. Transfection with a miRNA and a GFP reporter
B. Curcumin treatment with 4 different concentrations and piperine
C. Wound Healing Assay (migration)
D. LC3 immunodetection (autophagy)
EXPERIMENTAL APPROACH
AIMS
Objective: show that curcumin + miRNA206 can block migration and increase autophagy of cancer cells
Specific aims:
- Effective concentration of curcumin
- Cell migration inhibition
- Autophagy induction
CONTEXT
- Cancer diseases
- Curcumin as an anticancer drug
- Inhibition of metastasis and PI3K/AKT/mTor pathway
ABSTRACT
COLLAB.
REFS.
Sup'Biotech Interactive Scientific Poster ©
BERAMICE Agyei, CHAMPOMIER Céline, FAYE Awa Thioub, LEGRAVEREND Clélia
Curcumin effects on migration and autophagy of A549 lung cancer cells